Water and acetonitrile were used as solvents A and B, respectively. The elution gradient was as follows: 0–2 min isocratic 55% B, 2–10 min gradient from 55 to 80% B, 10–15 min gradient from 80 to 55% B.
Water and acetonitrile were used as solvents A and B, respectively. The elution gradient wasas follows: 0–2 min isocratic 55% B, 2–10 min gradient from 55 to 80% B, 10–15 min gradient from 80 to 55% B.