For SSP determination, the mince (3g) made from fillets
was homogenized with 27 ml of 0.6 M KCl buffer (pH 7.0, 20 mM Tris-
HCl) and centrifuged at 20,000g at 4 °C for 30 min. The protein
concentration of the supernatant was determined using bovine serum
albumin as a standard