3.1.1. Zinc remaining in the supernatant
Maximum binding was achieved at pH 7 (for zinc concentration at 1 mg/g of lactose, zinc remaining in the supernatant was up to 16.6 mg/100 mL of total added zinc, whereas only 14.0 mg, 10.6 mg and 8.5 mg/100 mL at pH 8, 9 and 10, respectively). Complex preparation method was optimized by varying zinc concentration and keeping lactose concentration constant. Zinc solution from stock solution was added slowly to the lactose solution to attain required zinc concentration in the final solution (1–10 mg/g of lactose). The addition of zinc from 1 to 5 mg/g of lactose resulted in little loss of zinc in precipitate (0.5–4.3 mg/100 mL). This small amount of zinc which precipitated could be due to the lower utilization of NaOH to maintain the required pH level (7.0) and also lower lesser conversion of zinc sulphate to zinc hydroxide which is considered as insoluble form of zinc. Further, increase in addition of zinc concentration from 6 to 10 mg/g of lactose resulted in maximum loss (15.8–60.3 mg/100 mL) of zinc in precipitate.
3.1.1 3.1.1. Zinc remaining in the supernatant
Maximum binding was achieved at pH 7 (for zinc concentration at 1 mg/g of lactose, zinc remaining in the supernatant was up to 16.6 mg/100 mL of total added zinc, whereas only 14.0 mg, 10.6 mg and 8.5 mg/100 mL at pH 8, 9 and 10, respectively). Complex preparation method was optimized by varying zinc concentration and keeping lactose concentration constant. Zinc solution from stock solution was added slowly to the lactose solution to attain required zinc concentration in the final solution (1–10 mg/g of lactose). The addition of zinc from 1 to 5 mg/g of lactose resulted in little loss of zinc in precipitate (0.5–4.3 mg/100 mL). This small amount of zinc which precipitated could be due to the lower utilization of NaOH to maintain the required pH level (7.0) and also lower lesser conversion of zinc sulphate to zinc hydroxide which is considered as insoluble form of zinc. Further, increase in addition of zinc concentration from 6 to 10 mg/g of lactose resulted in maximum loss (15.8–60.3 mg/100 mL) of zinc in precipitate.
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