The decolorization rate of wastewater was determined by
measuring the optical density at 600 nm. The spectrum
change of the filtered dyes effluent due to the generation
of MnP was recorded after 10 min with 7-day-old culture
filtrate from the stationary cultures, in the presence of
0.5 mM MnSO
4, 0.4 mM H
2O2
and 50 mM sodium mal-onate (pH 4.5). The spectrum change caused by the pro-duction of NsP was recorded after 10 min, in the presence
of 4 mM H
2O2and 50 mM sodium acetate buffer (pH
5.5), with the 7-day-old culturefiltrate from the stationary
cultures.