Secondly, The conidia of B. brassiana were produced by growing on PDA plates. The plates were then incubated at room temperature (25±2°C) under 40 watt fluorescent light 24 h. per day for 14-21 days. Conidia were harvested by flooding each plate with 3-5 ml of sterile distilled water add a drop of the surfactant Triton X 0.05 %, rubbing the surfact and filtration. Suspension of each variety of fungus were determined using an improved haemocytometer and adjusted to five concentrations 1×105, 1×106, 1×107, 1×108, 1×109 conidia/ml.