Flavonoids were measured using a colourimetric assay developed
by Jia, Tang, and Wu (1999), with some modifications. Total
flavonoid extraction was the same as described above. Then, 1 ml
of appropriately diluted sample was added to a 10 ml volumetric
flask containing 4 ml of deionised water. At time zero, 0.3 ml of
5% NaNO2 was added to each volumetric flask; at 5 min, 0.3 ml of
10% AlCl3 was added and at 6 min, 2 ml of 1 M NaOH was added.
Each reaction flask was then immediately diluted with 3.4 ml of
deionised water and mixed. After 30 min in the dark, absorbance
of the mixture was determined at 510 nm against a blank of deionised
water. The total flavonoid contents were then expressed as
rutin equivalents, in mg/100 g fresh sample.
Flavonoids were measured using a colourimetric assay developedby Jia, Tang, and Wu (1999), with some modifications. Totalflavonoid extraction was the same as described above. Then, 1 mlof appropriately diluted sample was added to a 10 ml volumetricflask containing 4 ml of deionised water. At time zero, 0.3 ml of5% NaNO2 was added to each volumetric flask; at 5 min, 0.3 ml of10% AlCl3 was added and at 6 min, 2 ml of 1 M NaOH was added.Each reaction flask was then immediately diluted with 3.4 ml ofdeionised water and mixed. After 30 min in the dark, absorbanceof the mixture was determined at 510 nm against a blank of deionisedwater. The total flavonoid contents were then expressed asrutin equivalents, in mg/100 g fresh sample.
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