The concentration of VEGF in rat hind paw was
determined using Murine VEGF ELISA Kit (BioVision).
Analysis of VEGF was performed using standard ELISA
methodology according to the kit’s manual. Briefly, paw
tissue was excised after 6 h of histamine-induced inflammation.
The tissues were homogenized in pre-chilled lysis
buffer containing protease inhibitor. The homogenate was
centrifuged and VEGF level was determined from the
supernatant obtained. All assays were performed in triplicate
and results were expressed in ng/mL protein.