Fish specimens were captured with a trawl at 5 localities in South Japan (35000N 139300E; 24000N 124000E; 35250N
139500E; 34400N 138200E; 23000N 124000E) in 2005 and 2006. Vouchers were deposited in National Research Institute of
Fisheries Sciences, Japan. Genomic DNA was extracted according to the protocol for Barcode of Life (Ivanova et al., 2006).