This was carried out by following
the kinetics of the oxidation of ABTS in the presence of H2O2, having
as a result a coloured product (oxidized ABTS) that could be
detected via absorption spectroscopy. In this way it was proven
that the biological activity of the Anti-HRP Abs attached to the surface
of the PC remained active. The washing process and the Ab-PC
selectivity towards HRP were found to be so effective that the
residual activity found in the solution with Ab Control PCs (i.e.,
with attached control Ab) was negligible.