The aquaculture wastewater was obtained from the culture of L.
calcarifer which is commonly known as Silver Sea Bass. It was
chosen because of their ability to survive inwater at various salinity
levels ranging from 0 ppt to 30 ppt. To ensure uniform wastewater
characteristics, 30 individuals of juvenile fish (standard body length
15e20 cm) were reared in a 500 L black polyethylene tank with
controlled temperature of 27 ± 2 C and continuous constant
supply of aeration. Same type of feed with 25% protein content was
given based on 3% of fish body weight to maintain continuous
release of phosphorus and nitrogen composition in the effluent.
Organic waste concentration was considered to be at its maximum
at the fifth day after every cycle of weekly 30% water exchange.
Weekly water exchange was necessary to avoid massive mortality
of the fish due to toxic compound accumulation. At this time, the
produced wastewater was sampled for the bioremediation purposes.
In this study, the sampled wastewater contained an average
of 5.59 ± 0.32 mg L1 ammonia, 0.125 ± 0.25 mg L1 nitrite,
12.22 ± 0.58 mg L1 nitrate and 6.75 ± 0.29 mg L1 phosphate.