Correlation between amylase activity and DNA concentration in
fresh saliva
Saliva samples were retrieved from 10 healthy male volunteers,
22–35-years old, at least 1 h after eating or drinking other
beverages than water. All samples were obtained at 10 am, since
amylase activity is known to vary during the day [14]. The amylase
activity was measured using the Phadebas Amylase test1 (Magle
Life Sciences) following the manufacturer’s instructions (Phadebas
Amylase test user manual, Magle Life Sciences, 2008). Three
replicates were analysed for each sample. One month after the first
analysis, new saliva samples were collected from volunteers #1, #2
and #10, and the amylase activity was re-measured, in triplicates.
Three replicates of 32 mL of saliva from each of the 10
volunteers were analysed for DNA concentration (see Sections
2.3.4 and 2.3.5). The correlation between salivary amylase activity
and salivary DNA concentration was investigated using Spearman’s
rank correlation test.
Correlation between amylase activity and DNA concentration infresh salivaSaliva samples were retrieved from 10 healthy male volunteers,22–35-years old, at least 1 h after eating or drinking otherbeverages than water. All samples were obtained at 10 am, sinceamylase activity is known to vary during the day [14]. The amylaseactivity was measured using the Phadebas Amylase test1 (MagleLife Sciences) following the manufacturer’s instructions (PhadebasAmylase test user manual, Magle Life Sciences, 2008). Threereplicates were analysed for each sample. One month after the firstanalysis, new saliva samples were collected from volunteers #1, #2and #10, and the amylase activity was re-measured, in triplicates.Three replicates of 32 mL of saliva from each of the 10volunteers were analysed for DNA concentration (see Sections2.3.4 and 2.3.5). The correlation between salivary amylase activityand salivary DNA concentration was investigated using Spearman’srank correlation test.
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