Briefly, 1 volume of sample (5 puL for red wine [1 1, v/v with methanol) was added to 1 volume of 2,2-diphenyl-1-picrylhydrazyl (DPPH) 0.094 mM in methanol up to completing 1 mL. The free radical scavenging activity using the free radical DPPH. reaction was evaluated by measuring the ab sorbance at 515 nm after 60 min of reaction at 20 oC in a spec trophotometer (Varian Cary 50, Victoria, Australia). The reaction was carried out in closed Eppendorf tubes shaken at 20 o C and the results were expressed as mmol/L Trolox equivalents (6-hidroxy- amethy carboxylic acid) (Sigma), a vitamin E analogue.