. Morphogenesis in vitro of P. alata. (a) Plant derived from stem segment cultured on ½ MSM medium supplemented with 10% coconut water for 60 days; (b) direct
organogenesis from internodal segment cultured on MSM medium supplemented with 8.8 M BA for 30 days; (c) indirect organogenesis obtained from internodal segment
cultured on MSM medium supplemented with 13.2 M BA for 90 days; (d) friable callus derived from nodal segment cultured on MSM medium supplemented with 20.7 M
plicoram for 60 days; (e) direct shoot formation from the midvein of leaf segment cultured with the adaxial surface in contact with medium supplemented with 13.2 M
BA, in the presence of light; (f) compact, non-morphogenic callus derived from leaf segment cultured with the abaxial surface in contact with medium supplemented with
13.2 M BA for 30 days. Bars = 1 cm.