Emulsions with good stability can be obtained by using a mixture of emulsifiers with preparation processes de- scribed in this paper. The formation of liquid crystal structure, however, shows strong dependence on the composition of formulation and preparation process [6, 7]. By optimizing the composition of formulation and preparation process, the liquid crystal emulsion can be formed. In the experiment, the formation process of the liquid crystal structure during preparation of emulsion was studied. The polarized microscope was utilized to monitor the sample during emulsification and homog- enization. Photos were taken under polarized light and at 10 min, 20 min after homogenization and the end of the preparation, respectively.