Diabetes Mellitus is a metabolic disorder characterized by high blood sugar level caused due to deficiency of
insulin secretion or insulin action. One of the therapeutic approach to treat Type II Diabetes is to lower the postprandial
blood glucose level by inhibition of carbohydrate hydrolyzing enzyme such as alpha-amylase. In present investigation
polyherbal formulation (PHF) composed of 22 medicinal plants having anti-diabetic property were selected from WHO
monographs and evaluated for in vitro alpha-amylase inhibitory activity. Air dried powders of 22 medicinal plants were
divided into four categories. One formulation named as(PHF1) was used and prepared by mixing the powders in an
optimized ratio of 80:10:5:5.Extracts of PHF1 prepared by soxhlet method using polar and non-polar solvent was subjected
to Inhibition assay by using Dinitro salicylic acid (DNS) method and phytochemical constituents in the extract was
analyzed qualitatively as well as by GC-MS.The results revealed the presence of glycosides, steroids, terpenoids, saponins,
phenols and tannins. In vitro study indicates that PHF1 Hot water extract showed maximum percentage inhibitionof alphaamylase
activity. This hot water extract of PHF1 can be effective in lowering postprandial hyperglycemia (PPHG)