Papaya fruit were harvested and processed as described in Section 2.1. Subsequently they were wounded (Section 2.4) and 20 L
suspension aliquots (1.0 × 10
8
cells/mL) of W. anomalus (strain
422) and M. guilliermondii (strain 443) were inoculated at the
injured sites prior (3, 12, and 24 h) and after (12 and 24 h),
respectively, inoculation with the C. gloeosporioides suspension
(1.0 × 10
5
conidia/mL). The fruit were incubated for 6 d after the
onset of the treatments, at 95% RH and 28
◦
C, and necrotic lesion
progression was daily assessed by measuring the diameter of the
necrotic area relative to the initial wound, and the percentage of
disease inhibition calculated using Eq. (1). Sterile distilled water
was used as negative control and the fungicide TECTO
®
(1000 ppm),
containing the active ingredient thiabendazole, as the positive