Pinus radiata surface-disinfested seeds were externally inoculated at doses of 104 or 106 spores/mL, stored at room temperature for one day until sowing in forest sowing 35-trays containing substrate composed of 80% peat plus 20% coconut fiber, and incubated at constant temperature 20 °C, and 12 h day/night light. Each treatment was applied to three trays, and trays were arranged in a randomized block design. The same conditions were repeated in a second experiment at the dose of 104 spores/mL.