The classic method for routine laboratory detection of thesehemoparasites in canine blood specimens is primarily based onmicroscopic examination of stained blood smears. The limita-tions of this method are that it requires a specially qualifiedperson to detect infections and also professional competence todifferentiate between parasites. Alternative improved methods,including immunological techniques and molecular studies, havebeen applied. Serological assays are approved fordetection; however, they suffer from cross-reactions and low sen-sitivity. Molec-ular detection methods for the differentiation of hemoparasitesinclude quantitative PCR, hybridization detection and the coupling andcomparison of molecular and serological assays for the detectionof the above-mentioned tick-borne hemopathogen species