The rheological behavior of the native gelatin and all cross-linked gelatin solutions was
characterized using a Bohlin CVOR-150 controlled stress rheometer (Bohlin Instruments Ltd.,
Gloucestershire, UK) using a cone-plate geometry (1 angle, 30 μm gap).
Shear viscosity of the 6.67% (w/v) gelatin solution was determined according to a method
modified from the BSI 757:1975 standard test method at 20C. The shear rate was fixed at 1 s-1. Flow
test of all samples was carried out at 25C in the shear rate range of 0.01 to 100 s-1
.
Temperature sweeps were run over the range from 40 to 10C and followed by temperature
ramp of 10 to 40C at a cooling/heating rate of 1C/min with constant strain amplitude of 10% chosen
from the linear viscoelastic region of the samples at 1Hz. Storage modulus (G) as a function of
heating temperature was recorded.