Moisture and ash were determined by gravimetric measure according to AOAC 935.29 and AOAC 923.03 (AOAC, 1995), respectively. The protein content was determined by Kjeldahl method (AOAC 920.53) using 5.95 as nitrogen-to-protein conversion factor (AOAC, 1995). Assessment of minerals (Fe and Zn) was made by Flame Atomic Absorption Spectroscopy after dry ashing, using an Atomic Absorption spectrophotometer Analyst 300 (Perkin Elmer).