To increase the protease recovery and purity, additional sodium citrate 10, 15 and 20% (w/w) was added to the first cycle top fraction from 70% (w/w) CE with 15% PEG2000-15% sodium citrate ATPS
To increase the protease recovery and purity,additional sodium citrate 10, 15 and 20% (w/w) wasadded to the first cycle top fraction from 70% (w/w) CEwith 15% PEG2000-15% sodium citrate ATPS