At higher calcium chloride additions (>~20 mM) and heating at ~70 °C, milk forms a coagulum with release ofwhey (Ramasubramanian et al. 2012). The nature and yield of the calcium-induced coagulum vary according to the heat treatment used. For example, preheating the milk at 90 °C for 10 min, which denatures most of the whey protein, max-imises the yield and also increases the hardness and storage modulus of the coagulum. However, UHT preheat treatment,which also denatures most of the whey protein, leads to avery soft coagulum and reduced protein yield (Ramasubra-manian et al. 2013). Addition of calcium chloride to milk causes a decrease in pH (for example, addition of 50 mMreduced the pH from 6 .6 to 5.8), but the coagulation was shown to be not solely acid-induced as coagulation still occurred, albeit with a reduced yield of coagulum, when the pH was readjusted to 6.6