Inoculation of explants: The surface sterilized meristem
tissue blocks were then prepared under
stereomicroscope by removal of outer tissue of meristem
with the help of sterile scalpel under aseptic condition
inside the laminar airflow cabinet until the length reached
to 2.0-1.0 cm of its base (Kalimuthu et al., 2007).
The individual meristematic dome of tissue was directly
inoculated to the tissue culture bottle containing MS
medium (Murashige and Skoog, 1962; Shajahan et al.,
2011) supplemented with a little concentration of adenine
for explants swelling and to attain its maximum size to
develop photoactive shoots which is required as per
treatment and the bottle were sealed with parafilm.