2. Materials and methods
2.1. Preparation of media
The metabolism of phenolic compounds by Lactobacillus spp.
was studied in cherry juice and broccoli puree. Cherry juice was
produced as described by Di Cagno et al. (2009), with some modifications.
In brief, frozen fruits were thawed, homogenized, treated
at 121 C for 10 min, and centrifuged at 10,000 g for 20 min.
Frozen inflorescences from broccoli were thawed and homogenized
to prepare broccoli puree. To avoid the inactivation of the endogenous
myrosinase, which hydrolyses glucosinolates into numerous
biologically active products, broccoli puree was not sterilized (Aires
et al., 2009). Both media were stored at 20 C before use.