The lysates were centrifuged and supernatants were removed. The microtubes were then placed in a microwave oven (with
specifications; LG grill, model No. MG-604AZ, input 220v-50/bHz,
microwave 1350 w, RF output 900 w,2,4500 MH) and heated twice
for 1 min at 900 W or three times for 1 min at 750 W. The pellets
were dissolved in 200 µl of TE and were extracted with an equal
volume of phenol/chloroform/ isoamyl alcohol (25:24:1) for 15 min.
The aqueous phase was recovered by centrifugation for 20 min and
precipitated with ethanol