ThzID1-M3 was formulated into calcium alginate pellets as previously
described. As controls for some
experiments, additional pellets were made that did not contain fungal
biomass. Fsp was grown in flasks with wheat straw, water, and
malt extract (20:20:1, w/w) at room temperature for 45 days with
12 h of light per day (1 day/night per day). Conidia were collected
from medium by filtration with 4 layers of cheesecloth, then centrifuged
for 10 min at 800
×
g. Conidia were washed with sterile
distilled water. The conidia concentration was determined using a
hemacytometer.