Animals, Diets, and Experimental Design
The study was performed in the metabolism barn at
the Lethbridge Research Centre. Eight ruminally-cannulated
beef heifers (average ± SD at the beginning of
the experiment; 451 ± 21 kg BW; 10.6 ± 5.6 kg DMI/d)
were used in a replicated 4 × 4 Latin square design with four 28-d periods.