In relation to LAB counts, our results are in accordancewith previous
studies that have shown that these bacteria are quite insensitive to
nitrite. Korkeala, Alanko, and Tiusanen (1992) observed in in vitro
studies in MRS broth added with different nitrite concentrations, that
LAB seemed to grow well at levels up to 100 mg/l, while a clear
reduction was found at 400 mg/l. Furthermore, heterofermentative
LAB are more sensitive than homofermentative ones (Dodds &
Collins-Thompson, 1984; Korkeala et al., 1992). LAB lack the ironsulfur
enzyme ferredoxin, which is inhibited by nitrite, and is involved
in ATP synthesis from pyruvate in other microorganisms such as
C. botulinum (Jay, 2000).
In relation to LAB counts, our results are in accordancewith previousstudies that have shown that these bacteria are quite insensitive tonitrite. Korkeala, Alanko, and Tiusanen (1992) observed in in vitrostudies in MRS broth added with different nitrite concentrations, thatLAB seemed to grow well at levels up to 100 mg/l, while a clearreduction was found at 400 mg/l. Furthermore, heterofermentativeLAB are more sensitive than homofermentative ones (Dodds &Collins-Thompson, 1984; Korkeala et al., 1992). LAB lack the ironsulfurenzyme ferredoxin, which is inhibited by nitrite, and is involvedin ATP synthesis from pyruvate in other microorganisms such asC. botulinum (Jay, 2000).
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