2.6. Analysis of phytochemical contents and antioxidant activity
Gamma oryzanol content in RBO was determined spectrophotometrically
(UVeVIS 1700, Shimadzu, Japan) following the method
of Mezouari and Eichner (2007), slightly modified. Briefly, RBO
samples were diluted in heptane. The content of gamma oryzanol
was determined by UV absorption at 315 nm gamma oryzanol,
contents in RBO were quantified against the standard curve. The
calibration curve was obtained with pure oryzanol in a concentration
range of 0e200 mg/L. The concentration of gamma oryzanol
was calculated and expressed as g/100 g oil.