Aflatoxins were extracted from rice grains following a previously
adopted method of Beltran et al. (2011), 20 gm of ground rice
was treated with 80 mL of mixture of acetonitrile–water (84:16) in
250 mL conical flask in an orbital shaker at 100 rpm for 90 min at
37 C. Then filtered through Whatman filter paper No. 4 and concentrated
by evaporating solvent at 50 C under reduced pressure