Elevated plus maze (Pellow et al. 1985) Each animal was
placed in the EPM for 10 min. The percentage of time spent
in the open arms (%Time OA: the time spent in open arms
divided by the time spent in both open and closed arms) and
the percentage of open arms entries (%Entries OA: the number
of entries in open arms divided by the number of entries in
both open and closed arms) were used as anxiety measures
(Rodgers and Dalvi 1997). Considering that grooming has
been shown to be sensitive to stress and to both anxiogenic
and anxiolytic drugs (Stilwell and Fentress 2010), the number
of grooming events was also quantified and used to assess
anxiety-like behavior. The number of closed arms entries
(entries CA) was used as a measure of locomotor activity
(Rodgers and Dalvi 1997). All variables were scored using
the video images of the tests and observers were blind to
group assignment.