Effect of equilibrium temperature and incubation time
Equilibration temperature and incubation time are two
important parameters in cloud point extraction. The effect of
equilibration temperature on the extraction efficiency of carmine
was optimized in the range of 40–70 C. The results in Fig. 3 illustrated
the extraction efficiency was increased with an increase in
temperature from 40 to 55 C and then decreased. Hence, 55 C
was selected as the optimum temperature in this study.
In order to obtain acceptable extraction efficiency the incubation
time of sample solution was investigated in the range of 10–
35 min. Fig. 4 shows an incubation time of 15 min is the best time
for maximum extraction.