Crude enzyme was used to treat grape slurry which contained
0.1 g L1 chlorothalonil at 40 C before fermentation. The Chd activity
was 14.50 U L1 and the amount added was 0.5 mL L1 slurry.
Different treat times (10 min, 30 min and 60 min) were applied to
test the elimination effect. Sample without addition of chlorothalonil
was performed as control. Sample without addition of
Chd was also studied. All experiments were carried out in triplicate.