2.2. Chemicalanalysisoffeed
Ingredients and diets were analyzed according to AOAC (2003) procedures including crude protein, calcium and total phosphorus. For the analysis of most amino acids, ingredients and diets were hydrolyzed in 6N HCl at 110◦C for 24h. The sulfur-containing amino acid content was measured after performic acid oxidation, and the tryptophan content was determined after alkaline hydrolysis (AOAC, 2003). Amino acid analyses were performed using high performance liquid chromatography (Hitachi L-8800 Amino Acid Analyzer, Tokyo, Japan).
2.2. ChemicalanalysisoffeedIngredients and diets were analyzed according to AOAC (2003) procedures including crude protein, calcium and total phosphorus. For the analysis of most amino acids, ingredients and diets were hydrolyzed in 6N HCl at 110◦C for 24h. The sulfur-containing amino acid content was measured after performic acid oxidation, and the tryptophan content was determined after alkaline hydrolysis (AOAC, 2003). Amino acid analyses were performed using high performance liquid chromatography (Hitachi L-8800 Amino Acid Analyzer, Tokyo, Japan).
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