Feed Analysis
Prior to feed manufacturing, zinc levels were analyzed
in critical feedstuffs (e.g., maize, wheat, soybean
meal, and monocalcium phosphate) and supplemental
zinc sources. Based on the analyses the experimental
diets were optimized according to the experimental setup
(Table 1). Thereafter, the zinc level was analyzed in
each of the experimental diets. Furthermore, the basal
diet was analyzed for moisture (ISO 6496), ash (NEN
3329), CP (ISO/CD 15670), crude fat (ISO DIS 6492),
crude fiber (ISO 6865:2001), calcium (ISO 6869:2001),
and phosphorus (ISO 6491:1999). All analysis in feedstuffs
and experimental diets were done by Schothorst
Feed Research. Analysis in the supplemental zinc sources
(zinc sulfate and organic zinc) was done by Zinpro
Corporation before the start of the experiment.