Crude plant extracts were investigated for phytotoxic activity against Lemna minor as per reported procedure.
Stock solutions at concentration of 30 mg/ml were prepared. For nourishment of Lemna minor, E-medium was prepared.
Test sample at concentration of 10, 100 and 1000 μg/ml was added to clean petri-plates.
The plates were left at room temperature to evaporate methanol. Afterwards, 20 ml of the Emedium was poured into plates. Sixteen healthy Lemna minor were selected and added to respective petri-plates.
The plates were incubated at 28oC in a growth chamber for one week. E-Medium without plant extract was