In order to identify a white colony-growing
microorganism in an LGI medium, coming from sugarcane stalk
apoplastic sap, some experiments were carried out in which
ML-318 variety was used. Then, apoplastic sap was extracted
and the microorganism of interest was isolated as well as some
morphological, cultural and biochemical tests were carried out,
which revealed 65 % probability that the microorganism of
interest belongs to Bacillus brevis. Nitrogenase activity was
measured by acetylene to ethylene reduction. Results also
indicate interaction between Acetobacter diazotrophicus and
the white colony-growing microorganism identified as Bacillus
brevis.