Production of valuable secondary metabolites through plant cell or organ culture is the best suited alternative to extraction of whole plant material and to increase production of secondary metabolites in in-vitro systems, feeding precursor or intermediate metabolites is an obvious and popular approach. The present investigation was aimed to study the influence of feeding of organic supplements, glycine (0-125 M), ferulic acid (0-200 M), phenylalanine (0-200 M), α-ketoglutaric acid (0-200 M) and pyruvic acid (0-200 M) on production of bacoside-A (a triterpenoid type secondary metabolite responsible for cognition effects) in shoot and callus biomass of Bacopa monniera (L.) Pennell. The shoots were raised in liquid Murashige and Skoog’s (MS) medium fortified with 5 M 6-benzyladenine (BA) and callus biomass on agar solidified MS medium containing 1 M 2,4-dichlorophenoxyacetic acid (2,4 -D) in conjunction with 5 M 1-napthaleneacetic acid (NAA). Among the organic supplements used, 100 M pyruvic acid effectively enhanced the production of bacoside-A in shoot as well as callus biomass. The bacoside-A content in in-vitro raised shoot biomass was 4.0 and 1.2 times higher as compared to control and shoot biomass of naturally grown plants respectively. Inclusion of pyruvic acid in MS medium for in-vitro shoot cultures of B. monniera, can be adapted for enhanced production of bacoside-A.