Fig. 7. Fluorescence imaging of live HeLa cells. (A) DIC image. (B) Initial fluorescence image, showing a minimal fluorescence signal, which was stable
for a period of 2 h (F). (C) Addition of 50 μM ZnPT. (D) Signal from MitoTracker Red. (E) Overlay of C and D. Pearson’s r = 0.64 ± 0.1 (n = 18). (G)
Quantification of the change in fluorescence signal intensity of DA-ZP1-TPP (black bars) compared with ZP1-TPP (gray bars) under similar conditions.
(Scale bar: 37 μm.)
Fig. 8. Decreased mitochondrial zinc uptake in cancerous cell lines. RWPE-1,
RWPE-2, and PC-3 prostate cell lines were incubated for 24 h in medium
supplemented with (+) and without (−) 50 μM ZnCl2. RWPE-1 is the only cell
line demonstrating a statistically significant (P < 0.001) increase in mitochondrial
zinc uptake when incubated in zinc-enriched media.
Chyan et al. PNAS | January 7, 2014 | vol. 111 | no. 1 | 147
BIOCHEMISTRY