Unless otherwise indicated, all experiments were performed as follows: 3 mL of reaction mixture was prepared containing 10 mM acetate buffer (pH 4.5), 25 mg L−1 MG and 0.5 U mL−1 laccase from the crude enzyme extracts. The reactions were carried out at 30 °C in the dark. The absorbance of the mixture at the maximum wavelength of MG (λmax 620 nm) was recorded at 0.5, 1–6 and 8 h. Decolorization was calculated according to the following formula: decolorization (%) = A0 − A/A0*100%, where A0 was the initial absorbance and Awas the final absorbance. Control samples were run in parallel containing the reaction mixture with the same amount of heat-denatured laccase.