2.2. Instrumentation
The HP-5MS capillary column (Agilent Technologies, Santa
Clara, CA, USA) was employed for chromatographic separation.
An HP-7890A gas chromatograph equipped with a MSD-
5975C mass spectrometer (Agilent Technologies) was used for
GCeMS analysis. The shaker was a SA31 from Yamato Scientific
Co. (Tokyo, Japan). A CR21G III high-speed refrigerated
centrifuge (Hitachi, Tokyo, Japan) was used. A DC400H ultrasonic
bath was provided by Delta (Taipei, Taiwan). The HPLC
system employed was a Shimadzu LC-20AT with a SPD-20A
UV-VIS detector (Shimadzu, Kyoto, Japan). The column used
for HPLC analysis was an Agilent XDB-C18 (4.6 150mm, 5 mm).