2.4. Fermentation conditions
The fermentations were carried out in glass jars with a working
volume of 2 L (including the inoculum). The substrate comprised of
hydrolyzed henequen leaf juice diluted to 6, 8 and 10 degrees Brix
(Bx) with distilled water. The Brix scale is used in the sugar and
winemaking industry to indicate the percent of sugar by weight
in a solution, in our case the soluble solids present in the henequen
juice were not only sugars but also other compounds not yet identified
(organic acids, saponins among others).