In each run, a 75 μL aliquot of supernatant of the samples and eight replicates from 0.15 to 10μgmL−1 DNA (calf-thymus, Sigma-Aldrich D4522) and from 0.15 to 18 μg mL−1 RNA (bakers-yeast, Sigma-Aldrich R7125) were transferred to 96-well microplates (Nunc).