Supercritical extraction and fractionation of natural compounds is one of the early and most studied applications in the field of supercritical fluids. Different process conditions for supercritical extraction had statistically significant influence on different mass concentrations of tocopherols, while fatty acid profile stayed almost unchanged. Compared to other extraction methods, using supercritical CO2 the lowest concentrations of pigments were obtained. By collecting hemp oil during supercritical CO2 extraction process it is possible to control the amount of extracted pigments. Hemp oil was extracted almost completely by supercritical CO2 and the obtained oil had higher to copherol content compared tocold pressing. Low temperatures of extraction, reduced energy consumption, high product quality and absence of solvent in extracts are numerous advantages than supercritical fluid extraction technique can provide as opposed to traditional methods of extraction.