Initial denaturating temperature: 94 °C for 9 min and then 23
thermal cycles for amplification in PCR reaction as: 94 °C for 1
min, 55 °C for 1 min and 72 °C for 50 s and final amplification
cycle (extension) at 72 °C for 2 min. Corrected programs of PCR
are shown in Table 1.