Pineapple has several beneficial properties including antioxidant activity. The fruit of pineapple was extracted
with ethyl acetate, methanol and water. The phenolic content of the extracts was determined by Folin–
Ciocalteu method and antioxidant activity was assayed through some in vitro models such as antioxidant
capacity by phosphomolybdenum, β-carotene-linoleate, and radical scavenging activity using α,α-diphenyl-
β-picrylhydrazyl (DPPH) method. The phenolic contents of the extracts as caffeic acid equivalents were found
to be highest in methanol (51.1%) followed by ethyl acetate (13.8%) and water extract (2.6%). Antioxidant
capacity of the extracts as equivalent to ascorbic acid (μmol/g of the extract) was in the order of methanol
extractNethyl acetate extractNwater extract. In comparison with butylated hydroxyanisole (BHA), at
100 ppm of concentration, the antioxidant and free radical scavenging activities of the extracts assayed
through β-carotene-linoleate and DPPH method were also found to be highest with methanol extract
followed by ethyl acetate and water extracts. The results indicated that the extent of antioxidant activity of the
extract is in accordance with the amount of phenolics present in that extract and the pineapple fruit being rich
in phenolics may provide a good source of antioxidant.
Pineapple has several beneficial properties including antioxidant activity. The fruit of pineapple was extractedwith ethyl acetate, methanol and water. The phenolic content of the extracts was determined by Folin–Ciocalteu method and antioxidant activity was assayed through some in vitro models such as antioxidantcapacity by phosphomolybdenum, β-carotene-linoleate, and radical scavenging activity using α,α-diphenyl-β-picrylhydrazyl (DPPH) method. The phenolic contents of the extracts as caffeic acid equivalents were foundto be highest in methanol (51.1%) followed by ethyl acetate (13.8%) and water extract (2.6%). Antioxidantcapacity of the extracts as equivalent to ascorbic acid (μmol/g of the extract) was in the order of methanolextractNethyl acetate extractNwater extract. In comparison with butylated hydroxyanisole (BHA), at100 ppm of concentration, the antioxidant and free radical scavenging activities of the extracts assayedthrough β-carotene-linoleate and DPPH method were also found to be highest with methanol extractfollowed by ethyl acetate and water extracts. The results indicated that the extent of antioxidant activity of theextract is in accordance with the amount of phenolics present in that extract and the pineapple fruit being richin phenolics may provide a good source of antioxidant.
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