The chemical AITC was obtained from Aldrich Chemical (>98%,Milwaukee, WI, USA).
GL-derived AITC was released from B. carinataDSM,
produced by mechanical oil extraction and appropriately formulated by a patented procedure able to modulate AITC release in time after watering.
The meal was characterized for its moisture content, residual oil and GL content as described in De Nicola et al.