The leaves were cut into small pieces and dried at 600C for 3 days. The dried leaves (500
g) were then grounded using Wiley laboratory mill and macerated in cold aqueous
ethanol (70% ethanol) for 48 hours. The extract was concentrated under reduced pressure
in a rotary evaporator at 400C and the concentrates dried at room temperature to yield
solid AML residues of approximately 5.1% w/w on dry weight basis.. The extract were
dissolved in 5% aqueous ethanol solution at desired concentration (10, 30, 100 and 300
mg/kg) just before use and administered orally 30 minutes prior to the administration of
inducers. Acetic acid, carrageenan and indomethacin (IND) were purchased from Sigma
Chemical Co.