The objective of this study was to compare the effects of dimethylformamide (DMF) and glycerol in canine (Canis lupus
familiaris) semen cryopreservation based on postthaw motility and velocity evaluated by computer-assisted sperm analysis (CASA)
and the effects on subjective progressive motility, percentage of live sperm, and plasma membrane functional integrity. The semen
was diluted in two steps with an egg-yolk Tris extender containing 6% glycerol or DMF, frozen in 0.25-mL straws, and stored in
liquid nitrogen. Immediately after thawing, samples were accessed for subjective sperm motility, sperm membrane functional
integrity, percentage of live sperm, and evaluation by CASA. There were differences (P < 0.05) between glycerol and DMF with
regard to subjective progressive motility (43.1% vs. 21.5%), objective progressive motility (11.8% vs. 6.2%), velocity average
pathway (31.1 vs. 23.1 mm/sec), and amplitude of lateral head (3.3 vs. 3.9 mm), which confirmed the efficiency of glycerol. In
conclusion, objective analysis performed by CASA confirmed that no benefits were derived by using DMF to replace glycerol for
cryopreservation of canine semen.
The objective of this study was to compare the effects of dimethylformamide (DMF) and glycerol in canine (Canis lupusfamiliaris) semen cryopreservation based on postthaw motility and velocity evaluated by computer-assisted sperm analysis (CASA)and the effects on subjective progressive motility, percentage of live sperm, and plasma membrane functional integrity. The semenwas diluted in two steps with an egg-yolk Tris extender containing 6% glycerol or DMF, frozen in 0.25-mL straws, and stored inliquid nitrogen. Immediately after thawing, samples were accessed for subjective sperm motility, sperm membrane functionalintegrity, percentage of live sperm, and evaluation by CASA. There were differences (P < 0.05) between glycerol and DMF withregard to subjective progressive motility (43.1% vs. 21.5%), objective progressive motility (11.8% vs. 6.2%), velocity averagepathway (31.1 vs. 23.1 mm/sec), and amplitude of lateral head (3.3 vs. 3.9 mm), which confirmed the efficiency of glycerol. Inconclusion, objective analysis performed by CASA confirmed that no benefits were derived by using DMF to replace glycerol forcryopreservation of canine semen.
การแปล กรุณารอสักครู่..