Leek leaf discs (1.6 cm diameter) were taken from
inner layers of leek plants using a punch and were placed
in glass Petri dishes (9 cm diameter).All essential oils
and compounds were suspended in distilled water plus
Triton X-100 (0.5%) as wetting agent. Because of the
poor water solubilityof 1,8-cineole an adequate amount
of ethanol was added to its suspension.